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This database is a collection of Olea europaea L. EST reads generated through 454 pyro-sequencing technology. Four different cDNA libraries were generated from fruits of a cultivar characterized by a high oleuropein (main terpene secoiridoid) content and an oleuropein-lacking natural variant, respectively at the beginning and at the end of fruit development. The database is constructed by first clustering (EST reads are searched pairwise against each other and grouped into clusters based on shared sequence similarity), then assembling (at high stringency) EST reads to produce into tentative consensus (TC) sequences and singletons (sESTs). The total number of unique transcripts found are annotated and classified according to their biological functions. Finally, differentially expressed genes are identifyed comparing the abundance of gene transcripts among different cDNA libraries.

Please, access to the database content

Sequencing:
Project managers:
Luciana Baldoni [IGV-CNR]
Gaetano Perrotta [ENEA]

 Bioinformatics:
Maria Luisa Chiusano [UniNa]: Project manager
Nunzio D'Agostino [UniNa]: Sequence analysis, database designer and webmaster
Contact us:     luciana.baldoni[AT]igv.cnr.it;     gaetano.perrotta[AT]trisaia.enea.it;     chiusano[AT]unina.it

Acknowledgements:

Disclaimer: whilst every effort has been taken to ensure the accuracy of the information and the reliability of the analyses available from this site, neither the sequencing group nor any of CAB group employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, or represents that its use would not infringe privately owned rights.
Created by NDAG∅